Read e-book online Antisense Technology, Part B PDF

By John N. Abelson, Melvin I. Simon, M. Ian Phillips

ISBN-10: 0080496717

ISBN-13: 9780080496719

ISBN-10: 012182215X

ISBN-13: 9780121822156

Antisense know-how is the facility to control gene expression inside mammalian cells supplying robust experimental techniques for the research of gene functionality and gene legislation. for instance, tools which inhibit gene expression allow reports probing the traditional functionality of a particular product inside of a phone. Such technique can be utilized in lots of disciplines resembling pharmacology, oncology, genetics, telephone biology, developmental biology, molecular biology, biochemistry, and neurosciences. This quantity should be a very vital software in biomedically-oriented research.The seriously acclaimed laboratory normal for greater than 40 years, equipment in Enzymology is among the such a lot hugely revered courses within the box of biochemistry. due to the fact 1955, each one quantity has been eagerly awaited, often consulted, and praised by way of researchers and reviewers alike. Now with greater than three hundred volumes (all of them nonetheless in print), the sequence comprises a lot fabric nonetheless suitable today-truly a necessary booklet for researchers in all fields of lifestyles sciences.

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W. Pasternak, Mol. Pharmacol. 50, 293 (1996). 34 J. Shen, S. Shah, H. Hsu, and B. C. Yoburn, Mol. Brain Res. 59, 247 (1988). 32 FIG. 7. Effect of icv administration of ODNs to different classes of Ͱ subunits of G proteins, and of ODNs directed to mRNAs encoding Ȑ and ͳ receptors, on the analgesia evoked by opioids at the supraspinal level. Animals were injected for 5 days with increasing amounts of the ODNs (see text). On day 6 the antinociceptive activity of opioids was evaluated in the thermal tail-flick test.

WATERMAN VOLUME 273. RNA Polymerase and Associated Factors (Part A) Edited by SANKAR ADHYA VOLUME 274. RNA Polymerase and Associated Factors (Part B) Edited by SANKAR ADHYA VOLUME 275. Viral Polymerases and Related Proteins Edited by LAWRENCE C. KUO, DAVID B. OLSEN, AND STEVEN S. CARROLL VOLUME 276. Macromolecular Crystallography (Part A) Edited by CHARLES W. , AND ROBERT M. SWEET VOLUME 277. Macromolecular Crystallography (Part B) Edited by CHARLES W. , AND ROBERT M. SWEET VOLUME 278. Fluorescence Spectroscopy Edited by LUDWIG BRAND AND MICHAEL L.

44 J. Lai, E. J. Bilsky, R. B. Rothman, and F. Porreca, Neuroreport 5, 1049 (1994). 45 R. B. Raffa, R. P. Martinez, and C. D. Connelly, Eur. J. Pharmacol. 258, R5 (1994). 46 G. C. Rossi, K. M. Sandifer, and G. W. Pasternak, Neurosci. Lett. 198, 99 (1995). 47 J. Garzo´n, M. Castro, and P. Sa´nchez-Bla´zquez, Analgesia 1, 4 (1995). 36 [1] ANTISENSE AND OPIOID RECEPTOR SIGNALING IN CNS 19 FIG. 8. Assignment of G proteins to Ȑ- and ͳ-opioid receptors in the production of supraspinal analgesia. Thick lines denote significant reductions in analgesic potency in mice undergoing treatment with ODNs to these G proteins.

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Antisense Technology, Part B by John N. Abelson, Melvin I. Simon, M. Ian Phillips

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